Valine metabolism Gluconeogenesis from 3-hydroxyisobutyrate
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چکیده
منابع مشابه
3-Hydroxyisobutyrate Dehydrogenase is involved in Valine and Isoleucine degradation in A. thaliana
Running title: 3-Hydroxyisobutyrate Dehydrogenase of Arabidopsis thaliana 1 2 3 To whom correspondence should be addressed: Hans-Peter Braun, Institut für Pflanzenge4 netik, Leibniz Universität Hannover, Herrenhäuser Str. 2, 30419 Hannover, Germany, Tel.: 5 +49 511 7622674, Fax: +49 511 76214351, mail: [email protected] 6 7 8 9 3-Hydroxyisobutyrate Dehydrogenase is involved in both,...
متن کاملPurification and Characterization of 3-Hydroxyisobutyrate Dehydrogenase from Rabbit
3-Hydroxyisobutyrate dehydrogenase (3-hydroxy2-methyl propanoate: NAD+ oxidoreductase, EC 1.1.1.31) was purified 1800-fold from rabbit liver by detergent extraction, differential solubility in polyethylene glycol and (NH4)%S04, and column chromatography on DEAE-Sephacel, phenyl-Sepharose, CM(carboxymethy1)-Sepharose, Affi-Gel Blue, and U1trogel AcA-34. The enzyme had a native Mr of 74,000...
متن کاملContribution of 3-hydroxyisobutyrate to the measurement
In this study we employed a capillary gas-liquid chromatographic-mass spectrometric (GLC-MS) method to measure the plasma concentrations of 3-hydroxybutyrate (3OHB) and 3-hydroxyisobutyrate (3-OHIB) in overnight fasted diabetic subjects and in normal subjects. Plasma contents of 3-hydroxybutyrate measured in this fashion were identical to those obtained by enzymatic assay using a commercial pre...
متن کامل3-Hydroxyisobutyrate dehydrogenase, an impurity in commercial 3-hydroxybutyrate dehydrogenase.
The enzymic determination of D-3-hydroxybutyrate and acetoacetate normally involves the use of 3-hydroxybutyrate dehydrogenase (HBDH, EC 1.1.1.30) of bacterial origin. We show that HBDH from Rhodopseudomonas spheroides (BCL, grade II) contains a 3-hydroxyisobutyrate dehydrogenase (HIBDH) activity: activity with 3-hydroxyisobutyrate as substrate was greater than 10% of that with 3-hydroxybutyrat...
متن کاملIsoleucine and Valine Metabolism in Escherichia coZi
On the basis of isotopic results obtained with Torulopsis utilis and Neurospora crassa, Strassman et al. (1) and Adelberg (2) have proposed a mechanism for valine biosynthesis in which pyruvate is converted to a-acetolactate. Acetolactate would then undergo a rearrangement to yield the carbon skeleton of valine. Abelson (3) showed that pyruvate, in competition with glucose, gave rise to the car...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1986
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2400909